ダイナミックリアクションセル^TM-誘導結合プラズマ質量分析法による糖尿病患者尿及び血清中の微量元素分析

Abstract

To assess whether differences in concentrations of trace elements between diabetics and controls can be regarded as significant，V，Cr，Ni，Cu，As and Se in urinary and serum samples from diabetics and healthy volunteers were determined by dynamic reaction cell (DRC™) inductively coupled plasma mass spectrometry. To obtain lower background of each element, DRC™ conditions such as ammonia used as reaction cell gas and rejection parameter q (RPq) were optimized for the measurement. This method with optimum conditions was validated by the analysis of standard reference materials of urine and serum. The results were in good agreement with the certified values. Urine samples supplied from patients (n = 29) with diabetes and from healthy volunteers (n = 11) were analyzed by this method, and the results were assessed by Students t-test at significance level of 0.05. As a result, no statistical significance was observed for urinary V, Ni, As and Se (P > 0.05). However, the concentrations of urinary Cu and Cr in the diabetic group were higher than those in healthy group (P < 0.05). On the other hand the concentration of Cr in serum (n = 19) from patients with diabetes was lower than reference value of serum Cr in controls.