キャピラリー電気泳動法による整腸薬の分離分析

Abstract

Atropine, berberine and acrinol were separated and analysed by capillary electrophoresis. Several modifiers such as sodium dodecylsulfate and ethanol were added to the electrophoretic buffer solution (20 mM sodium borate, pH 9.2) to improve the separation of the three compounds. These compounds were successfully separated within 13 min by using a 72 cm × 50 μm i.d. fused-silica capillary at30kV. The detection was done at 200 nm. The calibration graphs were straight lines over the range 0.5 - 2.0 × 10^<-4> mol 1^<-1> for each atropine, berberine and acrinol. RSDs were 1.7%, 1.9% and 1.3% for 1 × 10^<-4> mol 1^<-1> atropine, berberine and acrinol, respectively.